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G Protein-Coupled Receptor Signaling in Plants

Volume 1043 of the series Methods in Molecular Biology pp 37-43

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Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

  • Zhaoqing JinAffiliated withBiosciences Division, Oak Ridge National Laboratory
  • , Wellington MucheroAffiliated withBiosciences Division, Oak Ridge National Laboratory
  • , Jin-Gui ChenAffiliated withBiosciences Division, Oak Ridge National Laboratory

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Abstract

The heterotrimeric G-proteins form classical signal transduction complexes conserved in all eukaryotes. The repertoire of G-protein signaling complex is much simpler in plants than in metazoans. One of the best understood functions for the plant G-protein complex is its modulation of cell proliferation. A method is described here to quantify cell division and cell elongation in the Arabidopsis heterotrimeric G-protein mutants using the hypocotyl as a model system.

Key words

Arabidopsis Cell division Cell elongation Heterotrimeric G-proteins Hypocotyl