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Wound Regeneration and Repair

Volume 1037 of the series Methods in Molecular Biology pp 81-98

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Adult Stem Cells in Small Animal Wound Healing Models

  • Allison C. NautaAffiliated withHagey Laboratory for Pediatric and Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Institute of Stem Cell Biology and Regenerative Medicine, Stanford University School of MedicineDepartment of Surgery, Stanford University
  • , Geoffrey C. GurtnerAffiliated withHagey Laboratory for Pediatric and Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Institute of Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine
  • , Michael T. LongakerAffiliated withHagey Laboratory for Pediatric and Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Institute of Stem Cell Biology and Regenerative Medicine, Stanford University School of MedicineDepartment of Surgery, Stanford University

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Abstract

This chapter broadly reviews the use of stem cells as a means to accelerate wound healing, focusing first on the properties of stem cells that make them attractive agents to influence repair, both alone and as vehicles for growth factor delivery. Major stem cell reservoirs are described, including adult, embryonic, and induced pluripotent cell sources, outlining the advantages and limitations of each source as wound healing agents, as well as the possible mechanisms responsible for wound healing acceleration. Finally, the chapter includes a materials and methods section that provides an in-depth description of adult tissue harvest techniques.

Key words

Adult stem cells Adipose derived stromal cells Bone marrow derived mesenchymal cells