Protocol

Transplantation Immunology

Volume 1034 of the series Methods in Molecular Biology pp 319-329

Date:

A Flow Cytometric Crossmatch Test Using Endothelial Precursor Cells Isolated from Peripheral Blood

  • Annette M. JacksonAffiliated withImmunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine
  • , Donna P. LucasAffiliated withImmunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine
  • , Jessica L. BaddersAffiliated withImmunogenetics Laboratory, Department of Medicine, Johns Hopkins University School of Medicine

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Flow cytometric crossmatch tests provide a donor-specific, cell based method for the detection of alloreactive antibodies in the sera of transplant patients. Conventional crossmatch tests used in solid organ transplantation utilize lymphocytes as target cells to detect the presence of alloreactive HLA antibodies. Isolation of endothelial precursor cells (EPCs) from peripheral blood now allows testing for antibodies reactive with non-HLA endothelial cell antigens.

Key words

Endothelial cell Non-HLA antibodies Transplantation Flow cytometric crossmatch Alloantibodies