Abstract
Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.
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Acknowledgments
Research in the laboratory of JWdG is supported by grants from the Swedish Research Council, the Carl Tryggers Stiftelse, the Marianne and Marcus Wallenberg Foundation, NIH grant 5R01GM081827-03, and the SSF supported Center for Biomembrane Research. David Drew acknowledges the support of the Royal Society (UK) through a University Research Fellowship and the Swedish Research Council.
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Hjelm, A. et al. (2013). Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_24
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DOI: https://doi.org/10.1007/978-1-62703-487-6_24
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