Methods in Molecular Biology Volume 1033, 2013, pp 381-400
Date: 09 Aug 2013

Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions

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Abstract

Optimizing the conditions for the overexpression of membrane proteins in E. coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify the optimal expression intensity of a membrane protein using only one strain, and membrane proteins C-terminally fused to Green Fluorescent Protein (GFP) greatly facilitates the production of high-quality membrane protein material for functional and structural studies.