Antiviral Methods and Protocols

Volume 1030 of the series Methods in Molecular Biology pp 93-103


Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon

  • Inge VliegenAffiliated withRega Institute for Medical Research
  • , Leen DelangAffiliated withRega Institute for Medical Research
  • , Johan NeytsAffiliated withRega Institute for Medical Research

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The current standard of care (SOC) for hepatitis C virus (HCV) genotype 1-infected patients consists of telaprevir or boceprevir in addition to pegylated interferon and ribavirin treatment. Other selective inhibitors of HCV replication are being developed. Drug pressure may, depending on the class of inhibitors, (rapidly) select for drug-resistant variants. Here we describe four different approaches to select in vitro for drug-resistant HCV subgenomic replicons.

Key words

Drug resistance HCV Replicon Genotyping Phenotyping