Antiviral Methods and Protocols

Volume 1030 of the series Methods in Molecular Biology pp 59-79


In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis

  • Rumin ZhangAffiliated withIn Vitro Pharmacology, Merck Research Laboratories
  • , William T. WindsorAffiliated withIn Vitro Pharmacology, Merck Research Laboratories

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Kinetic profiling of drug binding to its target reveals important mechanistic parameters including drug–target residence time. In this chapter, we focus on global progress curve analysis as a convenient method for kinetic profiling. Detailed guidelines with pros and cons for various experimental designs and data analysis are provided. Kinetic profiling of Boceprevir and Telaprevir is illustrated.

Key words

HCV NS3 protease Binding kinetics Kinetic profiling Drug–target residence time Global progress curve analysis Boceprevir Telaprevir