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Mouse Models of Innate Immunity

Volume 1031 of the series Methods in Molecular Biology pp 37-40

Date:

Collecting Resident or Thioglycollate-Elicited Peritoneal Macrophages

  • Monika SchneiderAffiliated withInfectious and Inflammatory Disease Center, Sanford Burnham Medical Research Institute

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Abstract

Peritoneal macrophages are invaluable for gaining an understanding of innate immune responses due to their physiological relevance. These macrophages can be harvested from the peritoneum to give a resident population or can be elicited through the use of thioglycollate. This chapter describes how to collect each type of macrophage.

Key words

Primary macrophages Ex vivo Innate immunity