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Glycosyltransferases

Volume 1022 of the series Methods in Molecular Biology pp 41-50

Date:

Study of the Biological Functions of Mucin Type Core 3 O-glycans

  • Seung Ho LeeAffiliated withScripps Korea Antibody InstituteTumor Microenvironment Program, Cancer Center, Sanford Burnham Medical Research Institute
  • , Minoru FukudaAffiliated withScripps Korea Antibody InstituteTumor Microenvironment Program, Cancer Center, Sanford Burnham Medical Research Institute

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Abstract

Core 3 O-glycan is very short glycan structure which is composed of one N-acetylglucosamine and one N-acetylgalactosamine. The core 3 O-glycan structure is synthesized by core3 synthase (beta 1, 3-N-acetylglucosaminyltransferase 6) using UDP-N-acetylglucosamine as substrate. We revealed that the core 3 O-glycan structure modulates prostate cancer formation and gastrointestinal cell differentiation through regulating the heterodimerization of α2β1 integrin [1, 2] and cell surface expression of differentiation marker proteins [3] respectively. This chapter describes the way to determine the functions of core 3 O-glycan in tumor formation and gastrointestinal cell differentiation.

Key words

Core 3 O-glycan Core 3 synthase Integrin α2β1 Gastrointestinal cell differentiation sucrase isomaltase Dipeptidylpeptidase IV