The Myc Gene

Volume 1012 of the series Methods in Molecular Biology pp 213-219


Studying Myc’s Role in Metabolism Regulation

  • Anne LeAffiliated withDepartment of Pathology, Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins University School of Medicine
  • , Chi V. DangAffiliated withAbramson Cancer Center, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania

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The MYC oncogene encodes a master transcription factor, Myc, which regulates genes involved in ribosome biogenesis, lipid synthesis, nucleic acid synthesis, intermediary metabolism, and cell growth and proliferation. The genomics of Myc target genes has been well-established through global mapping of Myc binding sites in a variety of different cancer cell lines. These studies highlight the importance of Myc in regulating glucose and glutamine metabolism as well as mitochondrial and ribosomal biogenesis. These genomic studies, however, only become relevant with the companion metabolic studies using a variety of methods to measure oxygen consumption, glucose uptake, or metabolic pathways based on 13C-labeled glucose or glutamine uptake. These methods are described herein.

Key words

MYC Cancer metabolism Oxygen consumption Glucose uptake Stable isotope resolved metabolomics (SIRM) 13C-labeled glucose or glutamine