Protocol

Plant Lipid Signaling Protocols

Volume 1009 of the series Methods in Molecular Biology pp 79-91

Date:

Lipidomic Analysis of Plant Membrane Lipids by Direct Infusion Tandem Mass Spectrometry

  • Sunitha ShivaAffiliated withDivision of Biology, Kansas Lipidomics Research Center, Kansas State University
  • , Hieu Sy VuAffiliated withDivision of Biology, Kansas Lipidomics Research Center, Kansas State University
  • , Mary R. RothAffiliated withDivision of Biology, Kansas Lipidomics Research Center, Kansas State University
  • , Zhenguo ZhouAffiliated withK-INBRE Bioinformatics Core Facility, University of Kansas
  • , Shantan Reddy MarepallyAffiliated withK-INBRE Bioinformatics Core Facility, University of Kansas
  • , Daya Sagar NuneAffiliated withK-INBRE Bioinformatics Core Facility, University of Kansas
  • , Gerald H. LushingtonAffiliated withK-INBRE Bioinformatics Core Facility, University of Kansas
  • , Mahesh VisvanathanAffiliated withK-INBRE Bioinformatics Core Facility, University of Kansas
  • , Ruth WeltiAffiliated withDivision of Biology, Kansas Lipidomics Research Center, Kansas State University

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Abstract

Plant phospholipids and glycolipids can be analyzed by direct infusion electrospray ionization triple-quadrupole mass spectrometry. A biological extract is introduced in solvent by continuous infusion into the mass spectrometer’s electrospray ionization source, where ions are produced from the lipids. For analysis of membrane lipids, a series of precursor and neutral loss scans, each specific for lipids containing a common head group, are obtained sequentially. The mass spectral data are processed and combined, using the Web application LipidomeDB Data Calculation Environment, to create a lipid profile.

Key words

Phospholipid Galactolipid Lipidomics Precursor scan Neutral loss scan Electrospray ionization Triple quadrupole Mass spectrometry