Protocol

Necrosis

Volume 1004 of the series Methods in Molecular Biology pp 67-73

Analysis of Pyroptosis in Bacterial Infection

  • Lia DanelishviliAffiliated withDepartment of Biomedical Sciences, College of Veterinary Medicine, Oregon State University
  • , Luiz E. BermudezAffiliated withDepartment of Biomedical Sciences, College of Veterinary Medicine, Oregon State UniversityDepartment of Microbiology, College of Science, Oregon State UniversityProgram of Molecular and Cell Biology, Oregon State University

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Abstract

Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells.

Key words

Pyroptosis Apoptosis Necrosis Macrophages Methods Caspase-1 Inflammatory response