Methods in Molecular Biology Volume 1001, 2013, pp 289-298
Date: 18 Feb 2013

Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold

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As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.