Protocol

Organ Regeneration

Volume 1001 of the series Methods in Molecular Biology pp 289-298

Date:

Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold

  • Pedro M. BaptistaAffiliated withWake Forest Institute for Regenerative Medicine
  • , Dipen VyasAffiliated withWake Forest Institute for Regenerative Medicine
  • , Emma MoranAffiliated withWake Forest Institute for Regenerative Medicine
  • , Zhan WangAffiliated withWake Forest Institute for Regenerative Medicine
  • , Shay SokerAffiliated withWake Forest Institute for Regenerative Medicine

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Abstract

As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.

Key words

Human liver progenitors Decellularization Scaffold Bioengineering Endothelial cells Perfusion bioreactor Whole organ Recellularization