Protocol

Organ Regeneration

Volume 1001 of the series Methods in Molecular Biology pp 267-278

Date:

Bioengineered Skin Substitutes

  • Pedro LeiAffiliated withDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York
  • , Hui YouAffiliated withDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York
  • , Stelios T. AndreadisAffiliated withDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York

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Abstract

Bioengineered skin has great potential for use in regenerative medicine for treatment of severe wounds such as burns or chronic ulcers. Genetically modified skin substitutes have also been used as cell-based devices or “live bioreactors” to deliver therapeutics locally or systemically. Finally, these tissue constructs are used as realistic models of human skin for toxicological testing, to speed drug development and replace traditional animal-based tests in a variety of industries. Here we describe a method of generating bioengineered skin based on a natural scaffold, namely, decellularized human dermis and epidermal stem cells.

Key words

Epidermal stem cells Three-dimensional tissue constructs Keratinocytes Differentiation