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Capillary Electrophoresis of Biomolecules

Volume 984 of the series Methods in Molecular Biology pp 13-25

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Capillary Electrophoresis–Mass Spectrometry of Carbohydrates

  • Joseph ZaiaAffiliated withDepartment of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University Medial Campus Email author 

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Abstract

The development of methods for capillary electrophoresis (CE) with on-line mass spectrometric detection (CE/MS) is driven by the need for accurate, robust, and sensitive glycomics analysis for basic biomedicine, biomarker discovery, and analysis of recombinant protein therapeutics. One important capability is to profile glycan mixtures with respect to the patterns of substituents including sialic acids, acetate, sulfate, phosphate, and other groups. There is additional need for an MS-compatible separation system capable of resolving carbohydrate isomers. This chapter summarizes applications of CS/MS to analysis of carbohydrates, glycoproteins, and glycopeptides that have appeared since 2008. Readers are referred to recent comprehensive reviews covering earlier publications.

Key words

Capillary electrophoresis Mass spectrometry