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Pancreatic Cancer

Volume 980 of the series Methods in Molecular Biology pp 121-129

Date:

Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis

  • Alvin Makohon-MooreAffiliated withDepartment of Pathology, Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins Medical Institutions
  • , Christine A. Iacobuzio-DonahueAffiliated withDepartment of Pathology, Sol Goldman Pancreatic Cancer Research Center, Johns Hopkins Medical Institutions Email author 

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Abstract

Sequencing analyses have been invaluable in identifying the genes associated with pancreatic ­carcinogenesis. However, whereas gene discovery related to carcinogenesis can be fairly straightforward, there are several additional aspects of experimental design that need to be considered when performing genetic analyses of metastatic disease. This chapter aims to review these issues and provide examples of the types of data generated.

Key words

Metastasis Genetics Comparative lesion sequencing Pancreatic cancer