Volume 971 of the series Methods in Molecular Biology pp 27-47


Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma

  • David WuAffiliated withDepartment of Laboratory Medicine, University of Washington
  • , Brent L. WoodAffiliated withDepartment of Laboratory Medicine, University of Washington
  • , Jonathan R. FrommAffiliated withDepartment of Laboratory Medicine, University of Washington Email author 

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Multiparametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes a general approach for the identification of abnormal lymphoid populations by flow cytometry, including B, T, and Hodgkin lymphoma cells suitable for the clinical and research environment. Knowledge of the common patterns of antigen expression of normal lymphoid cells is critical to permit identification of abnormal populations at disease presentation and for minimal residual disease assessment. We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma.

Key words

B cells B-cell lymphoma Clonality Flow cytometry Hodgkin lymphoma Light chain restriction T cells T-cell lymphoma T-cell receptor V-beta repertoire analysis