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Synthetic Messenger RNA and Cell Metabolism Modulation

Volume 969 of the series Methods in Molecular Biology pp 235-246

Date:

Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies

  • Mustafa DikenAffiliated withTranslational Oncology and Immunology (TRON) at the Johannes Gutenberg University Mainz
  • , Sebastian KreiterAffiliated withTranslational Oncology and Immunology (TRON) at the Johannes Gutenberg University Mainz
  • , Abderraouf SelmiAffiliated withTranslational Oncology and Immunology (TRON) at the Johannes Gutenberg University MainzDivision of Translational and Experimental Oncology, Department of Internal Medicine II, Johannes Gutenberg University Medical Center
  • , Özlem TüreciAffiliated withGanymed Pharmaceuticals
  • , Ugur SahinAffiliated withTRON-Translational Oncology at the University Medical Center MainzIII. Medical Department, University Medical Center of the Johannes Gutenberg-University MainzRibological, Biontech AG Email author 

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Abstract

Synthetic antigen-encoding mRNA is increasingly exploited as a tool for delivery of genetic information of complete antigens into professional antigen presenting dendritic cells for HLA haplotype-independent antigen-specific vaccination against cancer. Two strategies for mRNA-based antitumor vaccination have emerged into the clinical setting. One is transfection of autologous dendritic cells with synthetic mRNA for adoptive transfer into the patient. The other is direct injection of naked synthetic mRNA. Both methods have proven to be feasible and safe and to elicit antigen-specific immune responses. The design of novel synthetic vaccines employing synthetic mRNA requires further in-depth investigation of its bioavailability and immune pharmacology. This chapter summarizes the state-of-art in this field and describes methods elementary for preclinical studies of mRNA-based antitumor vaccine protocols.

Key words

mRNA vaccination Dendritic cell Cancer immunotherapy Lymph node