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Cell Senescence

Volume 965 of the series Methods in Molecular Biology pp 373-382

Date:

Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence

  • Narendra WajapeyeeAffiliated withDepartment of Pathology, Yale University School of Medicine
  • , Sara K. DeiblerAffiliated withHoward Hughes Medical InstitutePrograms in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School
  • , Michael R. GreenAffiliated withHoward Hughes Medical InstitutePrograms in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School Email author 

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Abstract

RNA interference (RNAi) is a powerful research tool that can be used to turn off—or silence—the expression of a specific gene. In recent years, RNAi screening on a genome-wide scale has provided the opportunity to identify factors and pathways involved in complex biological processes in a systematic and unbiased manner. Here we describe a genome-wide RNAi screening strategy to identify genes that are required for a particular type of cellular senescence called oncogene-induced senescence. The approach we describe is a general screening strategy that can be applied to the study of other forms of cellular senescence, including replicative senescence.

Key words

Functional genomics Genome-wide Oncogene-induced senescence Positive-selection screen RNA interference siRNA shRNA