Methods in Molecular Biology Volume 962, 2013, pp 201-210

ChIP for Identification of p53 Responsive DNA Promoters

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Abstract

Chromatin immunoprecipitation assay (ChIP) has been frequently used to determine whether a transcriptional regulator can bind to a specific DNA element in the chromatin content of cells. Here, we describe a detailed protocol for this assay with hands-on tips based on our own experience in working on the transcriptional regulator and tumor suppressor p53.