Juxtacellular Neuronal Labelling, Physiological Characterization and Phenotypic Identification of Single Neurons In Vivo

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Abstract

Characterisation of the phenotype and physiological responsiveness of a neuron and identification of its innervation target(s) are important objectives for neuroscientists. This chapter describes the technique of juxtacellular neuronal labelling and presents procedures for the light and electron microscopic visualisation of labelled neurons and the identification of their neurochemical phenotypes and inputs. For those with little experience in extracellular neuronal recording, a detailed description of the required pieces of equipment and how they are configured is also provided.