Protocol

Mammalian Oocyte Regulation

Volume 957 of the series Methods in Molecular Biology pp 249-253

Date:

Counting Chromosomes in Intact Eggs

  • Teresa ChiangAffiliated withInstitute of Biomedical Sciences, Academia Sinica
  • , Michael A. LampsonAffiliated withDepartment of Biology, University of Pennsylvania Email author 

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Abstract

Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected.

Key words

Chromosome spread Chromosome count Ploidy Monastrol Kinetochores