Volume 954 of the series Methods in Molecular Biology pp 3-25


Identification of Legionella in the Environment

  • Natalia A. KozakAffiliated withCenters for Disease Control and Prevention Email author 
  • , Claressa E. LucasAffiliated withCenters for Disease Control and Prevention
  • , Jonas M. WinchellAffiliated withCenters for Disease Control and Prevention

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Legionella is ubiquitous in freshwater systems worldwide and can also be found in soil. Legionellosis may be caused by inhalation of aerosolized water or soil particles containing Legionella. Isolation of Legionella from the environment is an essential step in outbreak investigation and may also be performed within the context of a hazard analysis and control risk management plan. Culture remains the gold standard for detection of Legionella in environmental samples. Specific properties of environmental sites that could be a source of Legionella contamination, collection of samples from such sites, and procedures for culture of these samples for Legionella are described in this chapter.

Key words

Potable water Cooling tower Whirlpool spa Legionella culture BCYE agar Bulk water samples Biofilm swabs Acid treatment Filtration