Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery
- Yoshihisa Namiki
- … show all 1 hide
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Lipidic magnetic nanoparticles are a rapid and reliable magnet-guided nucleic acid delivery system, which we have named as “LipoMag” (Namiki et al., Nat. Nanotechnol 4:598–606, 2009). LipoMag is composed of a cationic lipid shell, an oleic acid binder, and an iron oxide nanocrystal core. Through the electrostatic force, positively charged LipoMag and negatively charged nucleic acid form complexes, termed “mag-lipoplex.” Under a magnetic field, LipoMag bearing nucleic acid can be controllably accumulated in target cells. Here we describe the procedure for the preparation of LipoMag which can be used for the transfection of nucleic acids, such as plasmid DNA and small interfering RNA.
Supplementary Material (0)
- Namiki Y, Namiki T, Yoshida H, Ishii Y, Tsubota A, Koido S, et al. A novel magnetic crystal-lipid nanostructure for magnetically guided in vivo gene delivery. Nat Nanotechnol. 2009;4:598–606. CrossRef
- Namiki Y, Fuchigami T, Tada N, Kawamura R, Matsunuma S, Kitamoto Y, et al. Nanomedicine for cancer: lipid-based nanostructures for drug delivery and monitoring. Acc Chem Res. 2011;44:1080-93.
- Fuchigami T, Kawamura R, Kitamoto Y, Nakagawa M, Namiki Y. Ferromagnetic FePt-nanoparticles/polycation hybrid capsules designed for a magnetically guided drug delivery system. Langmuir. 2011;27:2923–8. CrossRef
- Fuchigami T, Kawamura R, Kitamoto Y, Nakagawa M, Namiki Y. A magnetically guided anti-cancer drug delivery system using porous FePt capsules. Biomaterials. 2012;33:1682–7.
- Namiki Y, Namiki T, Ishii Y, Koido S, Nagase Y, Tsubota A, et al. Inorganic-Organic Magnetic Nanocomposites for use in Preventive Medicine: A Rapid and Reliable Elimination System for Cesium. Pharm Res. 2012;29:1404–18.
- Kitamoto Y, He J. Chemical synthesis of FePt nanoparticles with high alternate current magnetic susceptibility for biomedicalapplications. Electrochim Acta. 2009;54:5969–72. CrossRef
- Koh I, Josephson L. Magnetic nanoparticle sensors. Sensors. 2009;9:8130–45. CrossRef
- Namiki Y, Namiki T. Synthesis of lipidic magnetic nanoparticles as nucleic acid and drug delivery system. Japanese Patent. 2008;4,183,047.
- Namiki Y. Sintered magnetic filter for the purifica-tion of magnetic nanoparticles. Japanese Patent Application. 2011;083367.
- Namiki Y, Matsunuma S, Inoue T, Koido S, Tsubota A, Namiki T, et al. Magnetic nanostructures for biomedical application. In: Masuda Y, editor. Nanocrystal. Rijeka, Croatia: Sciyo. 2011;349–372.
- Rosenweig RE. Magnetic fluids. Int Sci Tech. 1966;55:48–56.
- Reimers GW, Khalafalla SE. Production of magnetic fluids by peptization techniques. US Patent. 1974;3,843,540.
About this Protocol
- Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery
- Book Title
- Nanotechnology for Nucleic Acid Delivery
- Book Subtitle
- Methods and Protocols
- pp 243-250
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series Subtitle
- Methods and Protocols
- Series ISSN
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- Additional Links
- Lipidic magnetic nanoparticle (LipoMag)
- Gene therapy
- Plasmid DNA
- Small interfering RNA
- Industry Sectors
- Editor Affiliations
- ID1. , Department of Pharmacy, Ludwig-Maximilians-University Munich
- ID2. , Department of Pharmaceutical Sciences, Wayne State University
- Yoshihisa Namiki (0161)
- Author Affiliations
- 0161. Institute of Clinical Medicine and Research, The Jikei University School of Medicine, Kashiwa, Japan
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