Methods in Molecular Biology Volume 950, 2013, pp 293-311

Preparation of Gold Nanocluster Bioconjugates for Electron Microscopy

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Abstract

In this chapter, we describe types of gold nanoparticle–biomolecule conjugates and their use in electron microscopy. Included are two detailed protocols for labeling an IgG antibody with gold monolayer protected clusters. The first approach is a direct bonding approach that utilizes the ligand place exchange reaction. The second approach describes NHS–EDC coupling of Au144(pMBA)60 with IgG. Also included are various characterization techniques for determining labeling efficiency.