Detection of Mycoplasma Contaminations
- Cord C. UphoffAffiliated withDepartment of Human and Animal Cell Lines, DSMZ, German Collection of Microorganisms and Cell Cultures Email author
- , Hans G. DrexlerAffiliated withDepartment of Human and Animal Cell Lines, DSMZ, German Collection of Microorganisms and Cell Cultures and German Biological Resource Center
* Final gross prices may vary according to local VAT.Get Access
Mycoplasma contamination of cell lines is one of the major problems in cell culture technology. The specific, sensitive, and reliable detection of mycoplasma contamination is an important part of mycoplasma control and should be an established method in every cell culture laboratory. New cell lines as well as cell lines in continuous culture must be tested in regular intervals. The polymerase chain reaction (PCR) methodology offers a fast and sensitive technique to monitor all cultures in a laboratory and can also be used to determine the contaminating species.
The described assay can be performed in less than 3 hours, including sample preparation, DNA extraction, PCR run, and analysis of the PCR products. Special emphasis is given to steps taken to avoid false-negative results due to the presence of inhibitors of the Taq polymerase in the crude samples and the interpretation of the results. The technique can also be adapted to the requirements of the pharmacopoeia.
Key wordsBacteria Cell lines Contamination Mycoplasma PCR
- Detection of Mycoplasma Contaminations
- Book Title
- Basic Cell Culture Protocols
- pp 1-13
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series Subtitle
- Methods and Protocols
- Series ISSN
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- Additional Links
- Cell lines
- Industry Sectors
- eBook Packages
- Editor Affiliations
- ID1. , Experimental Therapeutics, BC Cancer Agency
- ID2. STEMCELL Technologies, Inc.
- Author Affiliations
- 00011. Department of Human and Animal Cell Lines, DSMZ, German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany
- 00012. Department of Human and Animal Cell Lines, DSMZ, German Collection of Microorganisms and Cell Cultures and German Biological Resource Center, Braunschweig, Germany
To view the rest of this content please follow the download PDF link above.