Volume 938 of the series Methods in Molecular Biology pp 109-113


Dienes’ Staining and Light Microscopy for Phytoplasma Visualization

  • Rita MusettiAffiliated withDepartment of Agricultural and Environmental Sciences, University of Udine Email author 

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The chapter describes the main light microscopy techniques used for the diagnosis of phytoplasmas. Because the described techniques are generally only effective in cases where the concentration of the pathogen inside the host phloem tissue is high, they are useful as preliminary methods to assess the presence of phytoplasmas and analyze the distribution in symptomatic plants.

Key words

Detection Dienes’ stain Light microscopy Phytoplasma