Methods in Molecular Biology Volume 935, 2013, pp 113-125

Generation of Transgenic X. laevis Models of Retinal Degeneration

Purchase on Springer.com

$49.95 / €39.95 / £34.95*

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Transgenic models are invaluable tools for researching retinal degenerative disease mechanisms. However, they are time-consuming and expensive to generate and maintain. We have developed an alternative to transgenic rodent models of retinal degeneration using transgenic Xenopus laevis. We have optimized this system to allow rapid analysis of transgene effects in primary transgenic animals, thereby providing an alternative to establishing transgenic lines, and simultaneously allowing rigorous comparisons between the effects of different transgenes.