Methods in Molecular Biology Volume 935, 2013, pp 215-226
Date: 22 Sep 2012

High-Throughput RNA In Situ Hybridization in Mouse Retina

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Abstract

The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.