Protocol

Retinal Degeneration

Volume 935 of the series Methods in Molecular Biology pp 215-226

Date:

High-Throughput RNA In Situ Hybridization in Mouse Retina

  • Seth BlackshawAffiliated withThe Salomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine Email author 

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Abstract

The introduction of large-scale gene expression profiling studies has greatly increased the need to rapidly obtain high-quality cellular expression patterns of genes found to exhibit differential expression. The use of large-scale nonradioactive RNA in situ hybridization makes this possible, and greatly increases the general usefulness of this data. Here, we describe protocols for parallel analysis of up to 50 different gene-specific probes in mouse retinal sections.

Key words

RNA Gene expression Cellular resolution Hybridization Digoxigenin Riboprobe Chromogenic Retina Photoreceptor Development