Methods in Molecular Biology Volume 935, 2013, pp 207-213
Date: 22 Sep 2012

Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL

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Terminal dUTP nick end labeling (TUNEL) is an invaluable technique used in the study of late-stage apoptosis. The technique is based upon detection of fragmented DNA, a well-recognized characteristic of apoptosis, usually with fluorescent markers. Here, we describe the TUNEL technique (1) employing two different detection techniques, fluorescence microscopy and fluorescence-activated cell sorting (2) which can be applied to the analysis of apoptosis in retinal tissues or retinal cell cultures, respectively.