Methods in Molecular Biology Volume 935, 2013, pp 207-213

Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL

Purchase on Springer.com

$49.95 / €39.95 / £34.95*

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Terminal dUTP nick end labeling (TUNEL) is an invaluable technique used in the study of late-stage apoptosis. The technique is based upon detection of fragmented DNA, a well-recognized characteristic of apoptosis, usually with fluorescent markers. Here, we describe the TUNEL technique (1) employing two different detection techniques, fluorescence microscopy and fluorescence-activated cell sorting (2) which can be applied to the analysis of apoptosis in retinal tissues or retinal cell cultures, respectively.