Methods in Molecular Biology Volume 923, 2013, pp 385-400

Quantification of Sporozoite Invasion, Migration, and Development by Microscopy and Flow Cytometry

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There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.