Protocol

Malaria

Volume 923 of the series Methods in Molecular Biology pp 385-400

Date:

Quantification of Sporozoite Invasion, Migration, and Development by Microscopy and Flow Cytometry

  • Photini SinnisAffiliated withDepartment of Molecular Microbiology and Immunology, John Hopkins Bloomberg School of Public Health Email author 
  • , Patricia De La VegaAffiliated withDepartment of Cellular Immunology, Walter Reed Army Institute of Research
  • , Alida CoppiAffiliated withRegeneron Pharmaceuticals
  • , Urszula KrzychAffiliated withDepartment of Cellular Immunology, Walter Reed Army Institute of Research
  • , Maria M. MotaAffiliated withMalaria Unit, Instituto de Medicina Molecular

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Abstract

There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.

Key words

Invasion Attachment Cell traversal Exoerythrocytic stages Sporozoites Malaria Plasmodium Flow cytometry