Protocol

Rational Drug Design

Volume 928 of the series Methods in Molecular Biology pp 185-195

Date:

Utilizing AntagomiR (Antisense microRNA) to Knock Down microRNA in Murine Bone Marrow Cells

  • Chinavenmeni S. VeluAffiliated withImmunobiology Division, Cincinnati Children’s Hospital Medical Center
  • , H. Leighton GrimesAffiliated withImmunobiology Division, Cincinnati Children’s Hospital Medical Center Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

MicroRNAs (miRNAs) are highly conserved small RNAs which regulate gene expression primarily through base pairing to the 3′ untranslated region of target messenger RNA (mRNA), leading to mRNA degradation or translation inhibition depending on the complementarity between the miRNA and target mRNA. Single miRNA regulates multiple target mRNA. miRNAs have been shown to regulate gene expression in the hematopoietic stem cells, as well as at key decision points for various lineages. However, aberrant expression of miRNAs has been documented in cancer and disease models. Rigorous dissection of miRNA pathways and biology requires facile loss of function modeling. This chapter describes detailed protocol for knockdown miRNA-21 which is involved in myelopoiesis using antagomiRs in primary murine bone marrow stem/progenitor cells.

Key words

microRNA Antagomir Murine bone marrow cells Drug delivery