Helicobacter Species

Volume 921 of the series Methods in Molecular Biology pp 11-15


Perspectives on Methodology for In Vitro Culture of Helicobacter pylori

  • Timothy L. CoverAffiliated withDepartment of Medicine and Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine Email author 

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Over the past 25 years, a variety of methods have been developed for culture of Helicobacter pylori in vitro. H. pylori is a capnophilic and microaerophilic organism that is typically cultured using complex culture media. Analysis of H. pylori growth in chemically defined media has provided insight into the nutritional requirements, physiology, and metabolic capacities of this organism.

Key words

Helicobacter pylori Defined medium Capnophilic Microaerophilic Nutritional ­requirements Nutrient acquisition