Protocol

DNA Repair Protocols

Volume 920 of the series Methods in Molecular Biology pp 149-162

Date:

Eukaryotic DNA Mismatch Repair In Vitro

  • Fenghua YuanAffiliated withDepartment of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine Email author 
  • , Limin SongAffiliated withDepartment of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine
  • , Fengsong LiuAffiliated withCollege of Life Sciences, Hebei University
  • , Liya GuAffiliated withGraduate Center for Toxicology, University of Kentucky College of Medicine
  • , Yanbin ZhangAffiliated withDepartment of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine Email author 

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Abstract

Mismatch repair corrects biosynthetic errors generated during DNA replication. Mismatch repair deficiency causes a mutator phenotype and directly underlies hereditary nonpolyposis colorectal cancer and some sporadic cancers. Because of remarkably high conservation of the mismatch repair machinery between the budding yeast (Saccharomyces cerevisiae) and humans, the study of mismatch repair in yeast has provided tremendous insights into the mechanisms of this repair pathway in humans. Here we describe a set of practical protocols for how to prepare the yeast and HeLa cell-free nuclear extracts and site-specific DNA mismatch substrates, and how to carry out the in vitro mismatch repair assay. We validated the yeast cell-free system by the mismatch repair deficient strain (Δmsh2) and the complementation assay with purified yeast MutSα.

Key words

In vitro DNA mismatch repair MMR Cancer Cell-free nuclear extract Yeast Saccharomyces cerevisiae Human