Protocol

Xenopus Protocols

Volume 917 of the series Methods in Molecular Biology pp 33-41

Date:

Generating Diploid Embryos from Xenopus tropicalis

  • Florencia del VisoAffiliated withDepartment of Pediatrics and Genetics, Yale University School of Medicine
  • , Mustafa KhokhaAffiliated withDepartment of Pediatrics and Genetics, Yale University School of Medicine Email author 

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Abstract

A spectacular advantage of Xenopus tropicalis is the ease with which diploid embryos can be generated year round. By the simple administration of human chorionic gonadotropin, an investigator can generate many hundreds of synchronized embryos by in vitro fertilization or thousands of embryos from a mating pair. The ability to induce ovulations when desired facilitates many different experiments such as experimental embryology, molecular manipulation of gene products, and genetics.

Key words

Xenopus tropicalis Egg Sperm Embryo In vitro fertilization hCG Cysteine