Abstract
Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu’s procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.
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Acknowledgment
This work was supported by the Serbian Ministry of Education and Science (grants 173028 and 173040).
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Maksimović, J.J.D., Živanović, B.D. (2012). Quantification of the Antioxidant Activity in Salt-Stressed Tissues. In: Shabala, S., Cuin, T. (eds) Plant Salt Tolerance. Methods in Molecular Biology, vol 913. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-986-0_16
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DOI: https://doi.org/10.1007/978-1-61779-986-0_16
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