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Nanoparticles in Biology and Medicine

Volume 906 of the series Methods in Molecular Biology pp 415-422

Date:

Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells

  • Linda BowmanAffiliated withPathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health
  • , Vincent CastranovaAffiliated withPathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health
  • , Min DingAffiliated withPathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health Email author 

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Abstract

Comet assay provides a rapid and reliable way to screen genotoxic effects of a wide variety of nanoparticles. Single cell gel electrophoresis assay (SCGE) or comet assay gained in popularity as a standard technique and is widely used for testing novel particles or chemicals for genotoxicity, monitoring environmental contamination with genotoxins, human biomonitoring and molecular epidemiology, and fundamental research in DNA damage and repair. Here, we describe the method of alkaline comet assay to detect nanoparticle-induced DNA damage.

Key words

Nanoparticles DNA damage Genotoxicity Reactive oxygen species Comet assay