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Nanoparticles in Biology and Medicine

Volume 906 of the series Methods in Molecular Biology pp 239-252

Date:

Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging

  • JasminAffiliated withInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de JaneiroDepartment of Neuroscience, Albert Einstein College of Medicine Email author 
  • , Ana Luiza Machado TorresAffiliated withInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro
  • , Linda JelicksAffiliated withDepartment of Physiology and Biophysics, Albert Einstein College of Medicine
  • , Antonio Carlos Campos de CarvalhoAffiliated withInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de JaneiroDepartment of Neuroscience, Albert Einstein College of Medicine
  • , David C. SprayAffiliated withDepartment of Neuroscience, Albert Einstein College of Medicine
  • , Rosalia Mendez-OteroAffiliated withInstituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro

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Abstract

Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamagnetic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.

Key words

Stem cells Cell labeling Ferumoxides Superparamagnetic iron oxide nanoparticles Magnetic resonance imaging