Protocol

Diagnosis of Sexually Transmitted Diseases

Volume 903 of the series Methods in Molecular Biology pp 225-233

Date:

Protocol for the Use of Enzyme-Linked Hybridization Assays for Genital Ulcer Disease

  • Seweryn BialasiewiczAffiliated withSir Albert Sakzewski Virus Research Centre Email author 
  • , Ian M. MackayAffiliated withSir Albert Sakzewski Virus Research Centre
  • , David M. WhileyAffiliated withSir Albert Sakzewski Virus Research Centre
  • , Theo P. SlootsAffiliated withSir Albert Sakzewski Virus Research Centre

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Etiologic agents of genital ulcer disease include herpes simplex 1 and 2, Treponema pallidum pallidum, Haemophilus ducreyi, and Klebsiella granulomatis. The advent of PCR has allowed for more rapid and sensitive detection of microbial pathogens. In this protocol, we describe the simultaneous detection of these five pathogens and an internal control using a single-tube multiplex PCR and colorimetric enzyme-linked amplicon hybridization assay.

Key words

Herpes simplex Haemophilus ducreyi Treponema pallidum Klebsiella granulomatis Genital ulcer Enzyme-linked hybridization Colorimetric Multiplex PCR Detection