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Quantitative Methods in Proteomics

Volume 893 of the series Methods in Molecular Biology pp 85-100

Date:

Quantitative Mass Spectrometry-Based Proteomics: An Overview

  • Miroslav NikolovAffiliated withBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry
  • , Carla SchmidtAffiliated withBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry
  • , Henning UrlaubAffiliated withBioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry Email author 

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Abstract

In recent years, mass spectrometry has moved more than ever before into the front line of protein-centered research. After being established at the qualitative level, the more challenging question of quantification of proteins and peptides using mass spectrometry has become a focus for further development. In this chapter, we discuss and review the strategies and problems of the methods currently in use for the quantitative analysis of peptides, proteins, and finally proteomes by mass spectrometry. The common themes, the differences, and the potential pitfalls of the main approaches are presented in order to provide a survey of the emerging field of quantitative, mass spectrometry-based proteomics.

Key words

Mass spectrometry Proteomics Absolute quantification Relative quantification Label-free Stable heavy isotope Isotope label