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Developmental Toxicology

Volume 889 of the series Methods in Molecular Biology pp 15-24

Date:

Caenorhabditis elegans as a Model in Developmental Toxicology

  • Windy A. BoydAffiliated withBiomolecular Screening Branch, National Toxicology Program, National Institute of Environmental Health Sciences, NIH
  • , Marjolein V. SmithAffiliated withSRA International
  • , Jonathan H. FreedmanAffiliated withBiomolecular Screening Branch, National Toxicology Program, National Institute of Environmental Health Sciences, NIHLaboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, NIH Email author 

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Abstract

A number of practical advantages have made the nematode Caenorhabditis elegans a useful model for genetic and developmental biological research. These same advantages, along with conservation of disease and stress response pathways, availability of mutant and transgenic strains, and wealth of biological information, have led to the increased use of C. elegans in toxicological studies. Although the potential to study the mechanisms of developmental toxicology in C. elegans is promising, embryonic and larval growth tests to identify compounds that affect the nematode have remained the primary use of C. elegans in developmental toxicology. Here, we describe a C. elegans larval growth and development assay for medium- and high-throughput screening using the COPAS Biosort flow cytometer and provide descriptions of the data and subsequent analysis.

Key words

C. elegans High-throughput screen Growth and development COPAS Biosort Developmental toxicity