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Odontogenesis

Volume 887 of the series Methods in Molecular Biology pp 95-110

Date:

Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs

  • Harald OsmundsenAffiliated withDepartment of Oral Biology, University of Oslo Email author 
  • , Anne-Marthe JevnakerAffiliated withDepartment of Oral Biology, University of Oslo
  • , Maria A. LandinAffiliated withDepartment of Oral Biology, University of Oslo

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Abstract

The use of deoxyoligonucleotide microarrays facilitates rapid expression profiling of gene expression using samples of about 1 μg of total RNA. Here are described practical aspects of the procedures involved, including essential reagents. Analysis of results is discussed from a practical, experimental, point of view together with software required to carry out the required statistical analysis to isolate populations of differentially expressed genes.

Key words

mRNA Transcriptome Hybridization Fluorescence Cy3 Cy5 MA-plot Clustering .gal-file