Protocol

Kidney Development

Volume 886 of the series Methods in Molecular Biology™ pp 211-221

Date:

Laser Capture

  • S. Steven PotterAffiliated withDivision of Developmental Biology, Children’s Hospital Medical Center Email author 
  • , Eric W. BrunskillAffiliated withDivision of Developmental Biology, Children’s Hospital Medical Center

* Final gross prices may vary according to local VAT.

Get Access

Abstract

This chapter describes detailed methods used for laser capture microdissection (LCM) of discrete subpopulations of cells. Topics covered include preparing tissue blocks, cryostat sectioning, processing slides, performing the LCM, and purification of RNA from LCM samples. Notes describe the fine points of each operation, which can often mean the difference between success and failure.

Key words

Laser capture Laser capture microdissection Tissue purification Small sample analysis Gene expression profiling