Abstract
Immortalized cell lines derived from embryonic mouse kidneys are useful tools for exploring signaling pathways, morphogenetic mechanisms, and gene function in renal development: they also provide a means for efficient first-round screening of panels of small molecules intended to combat renal pathologies such as the development of cysts, and such cell line-based screening can allow a valuable reduction in the numbers of animals needed for a given line of research. This chapter presents a simple method for generating cell lines from the “Immortomouse,” which carries a temperature-sensitive SV40 antigen, under the control of an interferon-regulated promoter.
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This work was supported by NC3Rs grant G0700480.
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Tai, G., Hohenstein, P., Davies, J.A. (2012). Making Immortalized Cell Lines from Embryonic Mouse Kidney. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_15
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DOI: https://doi.org/10.1007/978-1-61779-851-1_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-850-4
Online ISBN: 978-1-61779-851-1
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