Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique
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Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.
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- Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique
- Book Title
- Cancer Epigenetics
- Book Subtitle
- Methods and Protocols
- Book Part
- Part III
- pp 329-339
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series Subtitle
- Methods and Protocols
- Series ISSN
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- Additional Links
- Industry Sectors
- Editor Affiliations
- ID1. School of Medicine, Saba University
- ID2. National Institutes of Health (NIH), Div. Cancer Control &, National Cancer Institute
- Author Affiliations
- 1. Haematology, Centre for Cancer Research and Cell Biology Belfast, Queen’s University Belfast, Belfast, Northern Ireland, UK
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