Methods in Molecular Biology Volume 863, 2012, pp 329-339
Date: 03 Feb 2012

Detecting DNA Methylation Using the Methylated CpG Island Amplification and Microarray Technique

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Abstract

Methylated CpG island amplification and microarray (MCAM) is a two-color array technique that quantifies DNA methylation by hybridizing equimolar amounts of treated vs. control cell-line DNA to an array platform. Sample preparation, hybridization, and scanning are performed over 1 week, but multiple samples can be prepared simultaneously, allowing for high-throughput processing and data acquisition. One microarray slide is required for each sample assayed, with a control sample hybridized with each test sample.