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Gene Synthesis

Volume 852 of the series Methods in Molecular Biology pp 197-213

Date:

In Silico Design of Functional DNA Constructs

  • Alan VillalobosAffiliated withDNA2.0, Inc.
  • , Mark WelchAffiliated withDNA2.0, Inc.
  • , Jeremy MinshullAffiliated withDNA2.0, Inc. Email author 

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Abstract

The promise of synthetic biology lies in the creation of novel function from the proper combination of genetic elements. De novo gene synthesis has become a cost-effective method for building virtually any conceptualized genetic construct, removing the constraints of extant sequences, and greatly facilitating study of the relationships between gene sequence and function. With the rapid increase in the number and variety of characterized and cataloged genetic elements, tools that facilitate assembly of such parts into functional constructs (genes, vectors, circuits, etc.) are essential. The Gene Designer software allows scientists and engineers to readily manage and recombine genetic elements into novel assemblies. It also provides tools for the simulation of molecular cloning schemes as well as the engineering and optimization of protein-coding sequences. Together, the functions in Gene Designer provide a complete capability to design functional genetic constructs.

Key words

Gene design Protein expression Synthetic biology Gene Designer Molecular cloning Molecular biology