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Volume 845 of the series Methods in Molecular Biology pp 19-39

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Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

  • Shantanu GangulyAffiliated withDepartment of Biological Sciences, Carnegie Mellon University
  • , Aaron P. MitchellAffiliated withDepartment of Biological Sciences, Carnegie Mellon University Email author 

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Abstract

Several gene disruption strategies have been described in Candida albicans to create homozygous mutants. We describe here a recyclable mini-blaster cassette containing C. albicans URA3 gene and 200-bp flanking repeats that is useful for disruption of C. albicans genes. The cassette can be used to create unmarked homozygous mutants which can be complemented at the HIS1 gene locus. This strategy of creating gene disruptions and subsequent complementation can be used to study gene function.

Key words

Genetics Mutants Complementation