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Leucocytes

Volume 844 of the series Methods in Molecular Biology pp 15-30

Date:

Detection and Quantification of Cytokines and Other Biomarkers

  • Evan L. ChiswickAffiliated withDepartment of Pathology and Laboratory Medicine, Boston University School of Medicine and Boston Medical Center
  • , Elizabeth DuffyAffiliated withDepartment of Pathology and Laboratory Medicine, Boston University School of Medicine and Boston Medical Center
  • , Brian JappAffiliated withDepartment of Pathology and Laboratory Medicine, Boston University School of Medicine and Boston Medical Center
  • , Daniel RemickAffiliated withDepartment of Pathology and Laboratory Medicine, Boston University School of Medicine and Boston Medical Center Email author 

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Abstract

Accurate measurement of cytokine concentrations is a powerful and essential approach to the study of inflammation. The enzyme-linked immunosorbent assay (ELISA) is a simple, low-cost analytical tool that provides both the specificity and sensitivity required for the study of cytokines in vitro or in vivo. This communication describes a systematic approach to develop an indirect sandwich ELISA to detect and quantify cytokines, or other biomarkers, with accuracy and precision. Also detailed is the use of sequential ELISA assays to analyze multiple cytokines from samples with limited volumes. Finally, the concept of a multiplex ELISA is discussed with considerations given to cost and additional time required for development.

Key words

Cytokines Sandwich ELISA Sequential ELISA Multiplex ELISA Antibodies