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Leucocytes

Volume 844 of the series Methods in Molecular Biology pp 183-187

Date:

Isolation and Differentiation of Monocytes–Macrophages from Human Blood

  • Dipti VijayanAffiliated withAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland Email author 

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Abstract

The prevalence of fungal infections remains high, and it is associated with significant mortality and morbidity. Macrophages are heterogeneous population of effectors enriched in regions of Candida colonization. These cells sense Candida, and are critical in the resolution of these infections. Here, we describe how macrophages are generated in the presence of colony-stimulating factor-1 (CSF-1); an important cytokine required for the survival, proliferation and ex-vivo differentiation of monocytes to macrophages.

Key words

Macrophages CSF-1 Candida infections Monocyte differentiation MACs separation