Protocol

Cardiovascular Development

Volume 843 of the series Methods in Molecular Biology pp 69-78

Date:

Whole-Mount Confocal Microscopy for Vascular Branching Morphogenesis

  • Yoh-suke MukouyamaAffiliated withLaboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health Email author 
  • , Jennifer JamesAffiliated withLaboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health
  • , Joseph NamAffiliated withLaboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health
  • , Yutaka UchidaAffiliated withLaboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health

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Abstract

We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.

Key words

Confocal microscopy Whole-mount immunohistochemistry Mouse embryo Blood vessel Lymphatic vessel Fluorescence Antibody Patterning