Cardiovascular Development

Volume 843 of the series Methods in Molecular Biology pp 21-28


Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting

  • Ching-Pin ChangAffiliated withDepartment of Medicine, Division of Cardiovascular Medicine, Stanford Cardiovascular Institute, Stanford University School of Medicine Email author 

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Formation of the cardiac outflow tract and great arteries involves complex morphogenetic processes, whose abnormities result in several clinically important diseases. Studies of these developmental processes are therefore important for understanding congenital vascular defects. However, the three-dimensional structure of arteries makes it challenging to analyze the pattern of vasculature using conventional histological approaches. Here we describe a vascular casting method to visualize the branching and connections of great arteries in developing embryos as well as in adult mice. This technique can be used to study the development of cardiac outflow tract, semilunar valves, and great arteries as demonstrated previously (Circ Res, 2008; Development 135: 3577–3586, 2008).

Key words

Great arteries Cardiac outflow tract Persistent truncus arteriosus Tetralogy of Fallot Overriding aorta Bicuspid aortic valve Pulmonic stenosis Heart valve Heart development Vascular patterning